6UP2
Crystal structure of the murine DHX36 helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.246, 67.691, 103.683 |
| Unit cell angles | 90.00, 92.49, 90.00 |
Refinement procedure
| Resolution | 45.392 - 1.970 |
| R-factor | 0.1822 |
| Rwork | 0.180 |
| R-free | 0.22910 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.285 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.392 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmeas | 0.057 | 0.643 |
| Number of reflections | 59446 | 5706 |
| <I/σ(I)> | 20.5 | 1.6 |
| Completeness [%] | 99.0 | 98.1 |
| Redundancy | 3.4 | 3 |
| CC(1/2) | 1.000 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 298 | 10% PEG3350, 200 mM proline, 100 mM HEPES, pH 7.5 |
