6UOQ

OsPYL/RCAR5 residues 24-29 solved from electron diffraction stills

Experimental procedure

Source type	ELECTRON MICROSCOPE
Source details	TEAM1
Temperature [K]	100
Detector technology	CMOS CAMERA
Collection date	2019-04-04
Detector	K2 IS
Wavelength(s)	0.0197
Spacegroup name	P 21 21 21
Unit cell lengths	4.720, 11.560, 39.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.474 - 1.007
R-factor	0.2358
Rwork	0.234
R-free	0.25570
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	7.474	7.474	1.200
High resolution limit [Å]	1.000	8.000	1.000
Rmerge	0.217		0.357
Rmeas	0.266		0.440
Total number of observations	2878
Number of reflections	1029		400
<I/σ(I)>	2.87		1.8
Completeness [%]	74.4		70.9
Redundancy	2.797		2.685
CC(1/2)	0.955		0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	10% ethanol