6UNT
Barrier-to-autointegration factor soaked in DMSO: 1 of 14 in MSCS set
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 277.15 |
| Detector technology | CCD |
| Collection date | 2011-08-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 41.770, 41.770, 213.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.994 - 1.750 |
| R-factor | 0.1715 |
| Rwork | 0.166 |
| R-free | 0.22260 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.210 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.101 | 1.075 |
| Rmeas | 0.105 | 1.116 |
| Rpim | 0.029 | 0.297 |
| Total number of observations | 276443 | |
| Number of reflections | 20320 | 1075 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 13.9 |
| CC(1/2) | 0.998 | 0.788 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277.15 | 80 mM Tris pH 8.5, 16% Ethanol, 10% PEG-1450 |
