6UN6

Crystal structure of green fluorescent protein (GFP); S65T, Y66(3-NO2Y); ih circular permutant (50-51)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	47.947, 67.719, 58.363
Unit cell angles	90.00, 102.28, 90.00

Refinement procedure

Resolution	38.530 - 1.500
R-factor	0.161
Rwork	0.159
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ofk
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13RC2_2986)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.528	1.540
High resolution limit [Å]	1.500	1.500
Rmerge	0.073	2.238
Number of reflections	57467	4040
<I/σ(I)>	16.7	1.11
Completeness [%]	98.1	93
Redundancy	13.26	12.56

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.15 M ammonium acetate, 34% PEG 3350