6UN4

Crystal structure of rsEGFP2, Y67(3-ClY), Y107(3-ClY)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.119499
Spacegroup name	P 21 21 21
Unit cell lengths	51.341, 61.848, 69.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.529 - 1.499
R-factor	0.1792
Rwork	0.178
R-free	0.19750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pfr
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13rc2_2986)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.529	34.529	1.540
High resolution limit [Å]	1.499	6.700	1.500
Rmerge	0.053	0.017	1.874
Rmeas	0.055	0.017	1.949
Number of reflections	35265	460	2510
<I/σ(I)>	24.27	106.23	1.32
Completeness [%]	98.1	98.5	95.4
Redundancy	13.247	12.348	13.119
CC(1/2)	1.000	1.000	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	293	80 mM HEPES, pH 8.1, 1.84 M ammonium sulfate