6UL3

Crystal structure of a lysozyme from Litopenaeus vannamei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-10-08
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	41.990, 59.321, 100.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.740 - 3.000
R-factor	0.2
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lys
RMSD bond length	0.005
RMSD bond angle	1.087
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17-3344-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.740	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	4921	4921
<I/σ(I)>	4.2
Completeness [%]	91.5
Redundancy	2.2
CC(1/2)	0.980	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	0.1M Tris pH 8.5, 25% (w/v) tert-Butanol