6UKG

HhaI endonuclease in Complex With DNA in space group P21 (pH 4.2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	61.849, 37.545, 69.923
Unit cell angles	90.00, 110.26, 90.00

Refinement procedure

Resolution	31.520 - 1.160
R-factor	0.1412
Rwork	0.141
R-free	0.15720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uke
RMSD bond length	0.007
RMSD bond angle	0.996
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.520	1.210
High resolution limit [Å]	1.160	1.160
Rmerge	0.093	0.354
Rpim	0.039	0.217
Number of reflections	192216	12298
<I/σ(I)>	14.5	3.6
Completeness [%]	88.9	66.5
Redundancy	6.1	3.2
CC(1/2)		0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	10% v/v 2-propanol, sodium phosphate / citric acid, pH 4.2, 0.2 M lithium sulfate