6UKC
Crystal structure of a lysozyme from Litopenaeus vannamei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-07-24 |
Detector | RIGAKU |
Wavelength(s) | 1.54 |
Spacegroup name | P 61 |
Unit cell lengths | 82.368, 82.368, 38.665 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.692 - 2.250 |
Rwork | 0.200 |
R-free | 0.24630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lsy |
RMSD bond length | 0.008 |
RMSD bond angle | 1.527 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.180 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 6270 | 6275 |
<I/σ(I)> | 8.2 | |
Completeness [%] | 86.2 | |
Redundancy | 2.8 | |
CC(1/2) | 0.996 | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298.15 | HEPES 0.1M, pH 7.5, 20 (w/v) PEG 10,000% |