6UJF
Crystal structure of the Clostridial cellulose synthase subunit Z (CcsZ) from Clostridioides difficile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.020, 46.530, 61.090 |
| Unit cell angles | 90.00, 94.50, 90.00 |
Refinement procedure
| Resolution | 31.692 - 2.000 |
| R-factor | 0.2369 |
| Rwork | 0.234 |
| R-free | 0.28170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uje |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.692 | 2.071 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.018 | 0.284 |
| Rmeas | 0.026 | 0.401 |
| Rpim | 0.018 | 0.284 |
| Number of reflections | 25294 | 2157 |
| <I/σ(I)> | 17.96 | 2.4 |
| Completeness [%] | 99.5 | 99.58 |
| Redundancy | 2 | 2 |
| CC(1/2) | 1.000 | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 100 mM MES, 200 mM calcium acetate, 20% (v/v) poly(ethylene glycol) 8000, 2.5 mM cellotriose |
