6UIO

Crystal structure of mouse CRES (Cystatin-Related Epididymal Spermatogenic)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1950
Spacegroup name	P 21 21 21
Unit cell lengths	51.048, 85.924, 111.677
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.530 - 1.830
R-factor	0.2282
Rwork	0.224
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6roa
RMSD bond length	0.016
RMSD bond angle	1.918
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.530	1.893
High resolution limit [Å]	1.827	1.827
Rmerge	0.082	0.842
Rmeas	0.096	0.971
Rpim	0.048	0.473
Number of reflections	39480	3967
<I/σ(I)>	17.15	2.22
Completeness [%]	88.8
Redundancy	4.1
CC(1/2)	0.996	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	292	2.1M (NH4)2SO4, 0.2M NaI