6UID

Structure of the cytoplasmic domain of the T3SS sorting platform protein PscD from P. aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 61 2 2
Unit cell lengths	48.086, 48.086, 235.509
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.999 - 1.450
R-factor	0.1699
Rwork	0.169
R-free	0.19830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a0e
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	MoRDa
Refinement software	PHENIX (1.14_3237)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.100	47.100	1.470
High resolution limit [Å]	1.450	7.940	1.450
Rmerge	0.069	0.038	0.912
Total number of observations	415491	3177	7489
Number of reflections	29928	272	1343
<I/σ(I)>	18.9	44.4	1.7
Completeness [%]	99.6	99.9	95.4
Redundancy	13.9	11.7	5.6
CC(1/2)	0.999	1.000	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	291	1.8 M sodium phosphate monobasic monohydrate, potassium phosphate dibasic