6UHO
Crystal Structure of C148 mGFP-cDNA-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-04-22 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.710, 52.220, 86.440 |
Unit cell angles | 90.00, 94.23, 90.00 |
Refinement procedure
Resolution | 64.530 - 1.950 |
R-factor | 0.2155 |
Rwork | 0.214 |
R-free | 0.25080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5n9o |
RMSD bond length | 0.010 |
RMSD bond angle | 1.783 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 86.204 | 53.592 | 2.060 |
High resolution limit [Å] | 1.950 | 6.170 | 1.950 |
Rmerge | 0.056 | 0.406 | |
Rmeas | 0.107 | 0.065 | 0.464 |
Rpim | 0.051 | 0.032 | 0.222 |
Total number of observations | 179992 | ||
Number of reflections | 41971 | 1414 | 6133 |
<I/σ(I)> | 9.4 | 15.7 | 3.3 |
Completeness [%] | 99.2 | 99.8 | 100 |
Redundancy | 4.3 | 4.1 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 1 microliter C148 mGFP-cDNA-2 (5 mg/mL (protein concentration) in 10 mM Tris Buffer pH 7.4, 137 mM NaCl) + 1 microliter crystallization condition (0.15 M potassium bromide, 30% (w/v) PEG MME 2000) in a sitting drop with a 70 microliter reservoir (0.15 M potassium bromide, 30% (w/v) PEG MME 2000) |