6UHH

Crystal Structure of Human RYR Receptor 3 ( 848-1055) in Complex with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-11
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97927
Spacegroup name	P 21 21 21
Unit cell lengths	62.043, 61.855, 279.128
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.521 - 3.138
R-factor	0.2372
Rwork	0.233
R-free	0.28740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uhb
RMSD bond length	0.004
RMSD bond angle	0.838
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.11.1-2575_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.190
High resolution limit [Å]	3.138	8.510	3.140
Rmerge	0.098	0.086	0.547
Rmeas	0.118	0.106	0.660
Rpim	0.065	0.061	0.363
Number of reflections	18902	965	919
<I/σ(I)>	6.6
Completeness [%]	96.0	86.1	97.7
Redundancy	3	2.7	3.1
CC(1/2)		0.940	0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	297	0.1 M Tris:HCl pH 8.5, 20% (w/v) PEG 1000, protein in co-crystallization with 10mM ATP