6UHE

Closed-form Crystal Structure of Human RYR Receptor 3 ( 848-1055)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97927
Spacegroup name	P 65
Unit cell lengths	64.673, 64.673, 469.779
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.340 - 2.892
R-factor	0.2561
Rwork	0.254
R-free	0.30160
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.938
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.11.1-2575_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.890	8.000	2.950
Rmerge	0.069	0.045	0.694
Rmeas	0.079	0.052	0.800
Rpim	0.038	0.025	0.391
Number of reflections	24385	1138	1111
<I/σ(I)>	8.1
Completeness [%]	98.2	93.7	99.2
Redundancy	4.1	4	3.8
CC(1/2)		0.986	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	297	0.1 M Tris:HCl pH 8.5, 1.2 M Sodium Citrate Tribasic