6UGI
Crystal structure of a fragment of E. coli tRNA(Asp) consisting of its acceptor stem/T stem-loop. Long unit cell.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.12720 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 43.519, 43.519, 281.795 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.380 - 1.750 |
| R-factor | 0.232 |
| Rwork | 0.231 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ugj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.913 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.380 | 1.872 |
| High resolution limit [Å] | 1.750 | 1.754 |
| Rmerge | 0.118 | 1.257 |
| Rmeas | 0.126 | 1.354 |
| Rpim | 0.045 | 0.494 |
| Number of reflections | 9226 | 3401 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 89.0 | 34.9 |
| Redundancy | 8.1 | 7.4 |
| CC(1/2) | 0.997 | 0.683 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 2 M ammonium sulfate |
