6UG5
Closed Dimer of Y77A Mutant Putative Ryanodine Receptor from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97927 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.012, 87.421, 74.041 |
Unit cell angles | 90.00, 110.56, 90.00 |
Refinement procedure
Resolution | 36.974 - 2.357 |
R-factor | 0.1765 |
Rwork | 0.174 |
R-free | 0.22410 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.116 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.400 |
High resolution limit [Å] | 2.357 | 6.400 | 2.360 |
Rmerge | 0.085 | 0.038 | 0.510 |
Rmeas | 0.095 | 0.042 | 0.593 |
Rpim | 0.042 | 0.018 | 0.296 |
Number of reflections | 36401 | 1890 | 1711 |
<I/σ(I)> | 11.2 | ||
Completeness [%] | 99.4 | 99.8 | 93.3 |
Redundancy | 5 | 5 | 3.7 |
CC(1/2) | 0.996 | 0.712 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 1.5M sodium citrate |