6UG4
Open Dimer of Y77A Mutant Putative Ryanodine Receptor from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 84.201, 84.201, 229.704 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.101 - 2.295 |
| R-factor | 0.1992 |
| Rwork | 0.197 |
| R-free | 0.23540 |
| Structure solution method | SAD |
| RMSD bond length | 0.050 |
| RMSD bond angle | 1.414 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.11.1-2575_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
| Rmerge | 0.111 | 0.081 | 0.725 |
| Total number of observations | 321203 | ||
| Number of reflections | 41451 | 2177 | 2074 |
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 96.2 | 90.3 | 98.1 |
| Redundancy | 7.7 | 8.9 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.1 M Bis-Tris Propane:NaOH pH 7.0, 2.5 M Ammonium Sulfate |
