6UFM
Cocrystal Structure of the Nocardia farcinica ileS T-box riboswitch in complex with its cognate tRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 A |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 42.230, 93.980, 179.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.990 - 2.820 |
| R-factor | 0.195 |
| Rwork | 0.191 |
| R-free | 0.23270 |
| Structure solution method | SAD |
| Starting model (for MR) | tRNA-Gly |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.742 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.990 | 2.920 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.080 | 1.960 |
| Number of reflections | 17899 | 1726 |
| <I/σ(I)> | 15.2 | 1.2 |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 13 | 10.3 |
| CC(1/2) | 0.994 | 0.712 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 20-25 % PEG 3350, 0.1 M Bis-Tris, pH 6.5, 0.2 M Li2SO4, 10 mM MgCl2, 1 mM Spermine |
