6UFE
The structure of a potassium selective ion channel at atomic resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | I 4 |
Unit cell lengths | 67.860, 67.860, 89.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.459 - 1.200 |
R-factor | 0.1331 |
Rwork | 0.133 |
R-free | 0.14770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ouf |
RMSD bond length | 0.030 |
RMSD bond angle | 4.090 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.230 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 61537 | 3673 |
<I/σ(I)> | 12.23 | |
Completeness [%] | 97.4 | |
Redundancy | 5.34 | |
CC(1/2) | 0.998 | 0.489 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | See paper for details |