6UEP
Structure of A. thaliana TBP bound to a DNA site with a C-C mismatch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.648, 46.654, 146.302 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 145.626 - 2.050 |
| R-factor | 0.1724 |
| Rwork | 0.172 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 145.626 | 2.080 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rpim | 0.025 | 0.267 |
| Number of reflections | 83131 | 1725 |
| <I/σ(I)> | 18.4 | 2.7 |
| Completeness [%] | 93.0 | |
| Redundancy | 3 | |
| CC(1/2) | 0.998 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M sodium acetate pH 4.5 and 3.2 M sodium formate |
