6UEJ

Crystal structure of human zinc finger antiviral protein bound to RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9762
Spacegroup name	P 65
Unit cell lengths	123.090, 123.090, 40.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.000 - 2.210
R-factor	0.213
Rwork	0.211
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u9g
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.668	40.668	2.340
High resolution limit [Å]	2.200	6.530	2.200
Rmerge	0.079	0.052	1.375
Rmeas	0.081	0.054	1.409
Total number of observations	371826
Number of reflections	18081	744	2858
<I/σ(I)>	20.69	58.11	2.08
Completeness [%]	99.8	99.3	98.6
Redundancy	20.564	18.96	20.803
CC(1/2)	0.999	0.999	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	7% Peg 8000, 30mM magnesium chloride, 10mM spermine, 50mM HEPES pH 7.5