6UD7

Crystal structure of full-length human DCAF15-DDB1(deltaBPB)-DDA1-RBM39 in complex with indisulam

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	81.032, 93.778, 264.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.100 - 2.300
R-factor	0.207
Rwork	0.205
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.390	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.248	3.598
Rmeas	0.262
Rpim	0.076
Number of reflections	90574	4436
<I/σ(I)>	6.1	0.6
Completeness [%]	99.8	98.7
Redundancy	11.8	11.7
CC(1/2)	0.993	0.483

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	2% (v:v) TacsimateTM, pH 5.0, 0.1 M sodium citrate tribasic dihydrate, pH 5.6, and 10-20% (w:v) polyethylene glycol 3350