6UCL
Transcription factor deltaFosB bZIP domain self-assembly, type-I crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9787 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 39.506, 51.956, 150.454 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.782 - 2.207 |
| R-factor | 0.2723 |
| Rwork | 0.271 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vpe |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.476 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
| High resolution limit [Å] | 2.190 | 5.940 | 2.190 |
| Rmerge | 0.059 | 0.038 | 0.845 |
| Rmeas | 0.066 | 0.045 | 0.934 |
| Rpim | 0.030 | 0.023 | 0.389 |
| Total number of observations | 21906 | ||
| Number of reflections | 4096 | 228 | 194 |
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 99.6 | 95 | 100 |
| Redundancy | 5.3 | 4.7 | 5.2 |
| CC(1/2) | 0.993 | 0.886 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 17% (w/v) PEG 4000, 0.2 M ammonium sulfate |
