6UB6
Crystal structure of a GH128 (subgroup IV) endo-beta-1,3-glucanase from Lentinula edodes (LeGH128_IV) in complex with laminaritetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.03318 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.871, 47.678, 52.549 |
| Unit cell angles | 90.00, 110.42, 90.00 |
Refinement procedure
| Resolution | 49.250 - 1.250 |
| Rwork | 0.174 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.272 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.330 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmeas | 0.109 | 1.302 |
| Number of reflections | 58956 | 9283 |
| <I/σ(I)> | 10.54 | |
| Completeness [%] | 99.1 | 98.2 |
| Redundancy | 5.9 | 5.6 |
| CC(1/2) | 0.999 | 0.659 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.6 M ammonium sulfate 5% dioxane 0.1 M MES pH 6.5 |
