6UB4
Crystal structure (C2 form) of a GH128 (subgroup IV) endo-beta-1,3-glucanase from Lentinula edodes (LeGH128_IV) in complex with laminaritriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.03316 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.775, 46.520, 114.786 |
| Unit cell angles | 90.00, 119.50, 90.00 |
Refinement procedure
| Resolution | 28.900 - 1.600 |
| R-factor | 0.1817 |
| Rwork | 0.180 |
| R-free | 0.20980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ub2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.693 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 92515 | 15138 |
| <I/σ(I)> | 12.36 | 1.46 |
| Completeness [%] | 96.7 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.999 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | ammonium sulfate 1.4 M 10% dioxane MMT 0.1 M |
