6UB1
Crystal structure of a GH128 (subgroup III) curdlan-specific exo-beta-1,3-glucanase from Blastomyces gilchristii (BgGH128_III) in complex with laminaribiose at -3 and -2 subsites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.458540 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.323, 60.804, 63.545 |
| Unit cell angles | 69.67, 78.24, 77.86 |
Refinement procedure
| Resolution | 35.490 - 1.600 |
| R-factor | 0.1623 |
| Rwork | 0.160 |
| R-free | 0.19690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6uay |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.945 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.490 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 87019 | 14093 |
| <I/σ(I)> | 13.35 | 7.48 |
| Completeness [%] | 86.2 | 86.8 |
| Redundancy | 3.3 | 3.2 |
| CC(1/2) | 0.994 | 0.986 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.05 M Potassium di-hydrogen phosphate, 24% Polyethylene glycol 8,000 |
