6UAY

Crystal structure of a GH128 (subgroup III) curdlan-specific exo-beta-1,3-glucanase from Blastomyces gilchristii (BgGH128_III)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.458810
Spacegroup name	P 1
Unit cell lengths	36.470, 48.720, 57.110
Unit cell angles	102.61, 89.78, 93.89

Refinement procedure

Resolution	47.430 - 1.800
R-factor	0.1733
Rwork	0.171
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uaq
RMSD bond length	0.015
RMSD bond angle	1.893
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.430	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	34823	2487
<I/σ(I)>	13.25	4.29
Completeness [%]	98.1
Redundancy	4.48
CC(1/2)	0.986	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.05 M Potassium di-hydrogen phosphate 20% Polyethylene glycol 8,000