6UAM
Apo-form Dimer of Y77A Mutant Putative Ryanodine Receptor from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.623, 89.993, 74.482 |
| Unit cell angles | 90.00, 110.89, 90.00 |
Refinement procedure
| Resolution | 37.806 - 2.802 |
| R-factor | 0.1923 |
| Rwork | 0.189 |
| R-free | 0.25310 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
| Rmerge | 0.105 | 0.052 | 0.665 |
| Total number of observations | 72329 | ||
| Number of reflections | 22375 | 1097 | 1135 |
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 98.4 | 93.2 | 99.7 |
| Redundancy | 3.2 | 2.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 273 | 1.5M sodium citrate, 0.1M Bis-tris |
