6U9Q
Crystal Structure Analysis of DNA-BCL11A Znf domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.000, 60.160, 75.790 |
| Unit cell angles | 90.00, 129.73, 90.00 |
Refinement procedure
| Resolution | 47.563 - 1.830 |
| R-factor | 0.1756 |
| Rwork | 0.175 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.563 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmeas | 0.048 | 1.250 |
| Rpim | 0.026 | 0.670 |
| Total number of observations | 5071 | |
| Number of reflections | 30114 | 1498 |
| <I/σ(I)> | 15.8 | 1 |
| Completeness [%] | 97.3 | 98.1 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | NaMalate~8.0 |
