6U9P
Wild-type MthK pore in ~150 mM K+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 63.811, 63.811, 44.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.050 - 1.650 |
| R-factor | 0.1852 |
| Rwork | 0.183 |
| R-free | 0.20750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ldc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.272 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.050 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.104 | 1.670 |
| Rmeas | 0.106 | 1.735 |
| Rpim | 0.022 | 0.467 |
| Number of reflections | 11414 | 1096 |
| <I/σ(I)> | 19.15 | |
| Completeness [%] | 99.9 | 98.92 |
| Redundancy | 23.2 | 13.5 |
| CC(1/2) | 0.998 | 0.653 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1:1 protein (in 100 mM potassium chloride, 10 mM MOPS-KOH, pH 8.0) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-KOH, pH 6.75 |
