6U8D
Crystal structure of hepatitis C virus IRES junction IIIabc in complex with Fab HCV2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-03-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 49.705, 161.223, 96.978 |
| Unit cell angles | 90.00, 102.66, 90.00 |
Refinement procedure
| Resolution | 61.362 - 1.807 |
| R-factor | 0.1746 |
| Rwork | 0.173 |
| R-free | 0.20880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Fab HAVx 6mwn |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.362 | 1.872 |
| High resolution limit [Å] | 1.807 | 1.807 |
| Rmerge | 0.040 | 1.134 |
| Rmeas | 0.047 | |
| Rpim | 0.023 | 0.669 |
| Number of reflections | 67168 | 6420 |
| <I/σ(I)> | 15.66 | 0.92 |
| Completeness [%] | 98.7 | 94.58 |
| Redundancy | 3.9 | 3.7 |
| CC(1/2) | 0.999 | 0.481 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.2 M ammonium acetate, 0.1 M bis-tris pH 5.5, 45% 2-methyl-2,4-pentanediaol |
