6U8D

Crystal structure of hepatitis C virus IRES junction IIIabc in complex with Fab HCV2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-03-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	C 1 2 1
Unit cell lengths	49.705, 161.223, 96.978
Unit cell angles	90.00, 102.66, 90.00

Refinement procedure

Resolution	61.362 - 1.807
R-factor	0.1746
Rwork	0.173
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Fab HAVx 6mwn
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.362	1.872
High resolution limit [Å]	1.807	1.807
Rmerge	0.040	1.134
Rmeas	0.047
Rpim	0.023	0.669
Number of reflections	67168	6420
<I/σ(I)>	15.66	0.92
Completeness [%]	98.7	94.58
Redundancy	3.9	3.7
CC(1/2)	0.999	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.2 M ammonium acetate, 0.1 M bis-tris pH 5.5, 45% 2-methyl-2,4-pentanediaol