6U89

RNA duplex, bound with TNA monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2017-06-09
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	51.892, 51.892, 37.891
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.960 - 2.360
R-factor	0.191
Rwork	0.188
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hbw
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.360	2.360
Rmerge	0.163	0.537
Rmeas	0.185	0.621
Rpim	0.086	0.307
Number of reflections	2444	228
<I/σ(I)>	10.67	1.89
Completeness [%]	99.9	99.6
Redundancy	4.5	3.9
CC(1/2)	0.933	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	25 % v/v Polyethylene glycol 400, 100 mM tri-Sodium citrate; pH 5.6, 130 mM Sodium chloride, 60 mM Magnesium chloride