6U7J

Uncultured Clostridium sp. Beta-glucuronidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	74.941, 105.655, 189.566
Unit cell angles	90.00, 94.60, 90.00

Refinement procedure

Resolution	29.310 - 2.200
R-factor	0.1654
Rwork	0.165
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jkm
RMSD bond length	0.008
RMSD bond angle	0.911
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.310	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	146335	14633
<I/σ(I)>	10.89
Completeness [%]	97.9
Redundancy	2.7
CC(1/2)	0.997	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Calcium Chloride, 0.1 M Tris: HCl, pH 8.5 and 20 % (w/v) PEG 4000