6U7G

HCoV-229E RBD Class V in complex with human APN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	99.555, 98.682, 147.525
Unit cell angles	90.00, 104.60, 90.00

Refinement procedure

Resolution	45.614 - 2.350
R-factor	0.1806
Rwork	0.179
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fyq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16rc1_3535)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	200.000	200.000	2.390
High resolution limit [Å]	2.349	6.380	2.350
Rmerge	0.052	0.024	0.377
Rmeas	0.062	0.028	0.459
Rpim	0.032	0.015	0.258
Number of reflections	114608	5863	5619
<I/σ(I)>	14.9
Completeness [%]	99.7	98.9	99
Redundancy	3.5	3.4	2.9
CC(1/2)		0.997	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	9% PEG8000, 1 mM oxidized glutathione, 1 mM reduced glutathione, 5% glycerol, 100 mM MES, pH 6.5