6U7B

Structure of E. coli MS115-1 CdnC:HORMA-deltaN complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	52.286, 142.897, 151.157
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	103.840 - 2.090
R-factor	0.2065
Rwork	0.205
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p80
RMSD bond length	0.003
RMSD bond angle	0.628
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	151.160	2.140
High resolution limit [Å]	2.090	2.090
Rmerge	0.081	1.305
Rmeas	0.089	1.414
Rpim	0.034	0.537
Number of reflections	67360	4202
<I/σ(I)>	16.2	1.4
Completeness [%]	99.5	94.1
Redundancy	6.6	6.6
CC(1/2)	0.999	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M CHES pH 9.5, 0.1 M magnesium acetate, and 17% PEG 3350