6U73

Human Angiopoietin-Like 4 C-Terminal Domain (cANGPTL4) with Myristic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978
Spacegroup name	C 2 2 21
Unit cell lengths	132.496, 135.632, 39.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.790 - 2.380
R-factor	0.1989
Rwork	0.194
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6eub
RMSD bond length	0.009
RMSD bond angle	1.104
Data reduction software	HKL-3000 (1.16_3549)
Data scaling software	HKL-3000 (1.16_3549)
Phasing software	AutoSol
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.790	2.470
High resolution limit [Å]	2.340	2.340
Rmerge	0.090	0.408
Rpim	0.039
Number of reflections	14821	712
<I/σ(I)>	17.2
Completeness [%]	96.4	99.7
Redundancy	6.3	6.5
CC(1/2)	0.985	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1M HEPES 7.5, 0.1M Sodium chloride, 1.6M Ammonium sulfate, Myristic acid