6U6Y
Human SAMHD1 bound to ribo(CGCCU)-oligonucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.340, 183.620, 81.560 |
| Unit cell angles | 90.00, 100.85, 90.00 |
Refinement procedure
| Resolution | 75.960 - 2.470 |
| R-factor | 0.1828 |
| Rwork | 0.180 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6u6x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.988 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.960 | 2.530 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.113 | 1.637 |
| Rpim | 0.068 | 0.995 |
| Number of reflections | 79766 | 5855 |
| <I/σ(I)> | 9.5 | 1.2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.995 | 0.499 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% PEG 4000, 0.6 M NaCl, 0.1 M MES:NaOH pH 6.5 |
