6U6N
Structure of the trimeric globular domain of Adiponectin mutant - D187A Q188A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | H 3 |
| Unit cell lengths | 75.704, 75.704, 48.865 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.180 - 2.150 |
| R-factor | 0.2142 |
| Rwork | 0.210 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6u66 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.433 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmeas | 0.730 | 0.660 |
| Number of reflections | 29889 | 865 |
| <I/σ(I)> | 12.42 | 1.59 |
| Completeness [%] | 99.1 | 94.4 |
| Redundancy | 5.3 | 3.04 |
| CC(1/2) | 0.990 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M sodium nitrate, 0.1 M MES pH 5.5, 20% PEG 3350 |
