6U69

Crystal structure of Yck2 from Candida albicans, apoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-12-11
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	H 3
Unit cell lengths	91.771, 91.771, 119.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.140 - 2.610
R-factor	0.1935
Rwork	0.191
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x18
RMSD bond length	0.004
RMSD bond angle	0.599
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3448)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.650
High resolution limit [Å]	2.610	2.610
Rmerge	0.067	0.360
Rpim	0.031	0.241
Number of reflections	11498	584
<I/σ(I)>	20.58	2.11
Completeness [%]	100.0	100
Redundancy	5.8	5.6
CC(1/2)		0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Tris pH 8, 25 mM magnesium chloride, 22% (w/v) PEG3350