6U52
Anti-Sudan ebolavirus Nucleoprotein Single Domain Antibody Sudan B (SB) Complexed with Sudan ebolavirus Nucleoprotein C-terminal Domain 634-738
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-10-06 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.977, 44.984, 66.438 |
| Unit cell angles | 90.00, 105.94, 90.00 |
Refinement procedure
| Resolution | 23.310 - 1.900 |
| R-factor | 0.2004 |
| Rwork | 0.198 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6u51 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.948 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.140 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.082 | 1.573 |
| Rpim | 0.044 | 0.849 |
| Number of reflections | 38124 | 5705 |
| <I/σ(I)> | 9.5 | 0.9 |
| Completeness [%] | 96.9 | 99.8 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.998 | 0.471 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 10% polyethylene glycol 6000, 1.0 M lithium chloride, 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.0 |
