6U50

Anti-Sudan ebolavirus Nucleoprotein Single Domain Antibody Sudan B (SB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-01-31
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54178
Spacegroup name	P 31 2 1
Unit cell lengths	47.839, 47.839, 85.378
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.270 - 1.600
R-factor	0.1736
Rwork	0.169
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6u51
RMSD bond length	0.009
RMSD bond angle	0.961
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.690	1.680
High resolution limit [Å]	1.600	1.600
Rmeas	0.142	1.820
Rpim	0.045	0.605
Number of reflections	15558	2140
<I/σ(I)>	12.1	1.3
Completeness [%]	99.4	96.2
Redundancy	9.7	8.5
CC(1/2)	0.999	0.367

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	37.5% precipitant mix (2-methyl-2,4-pentanediol, polyethylene glycol [PEG] 1000, PEG 3350), 0.1 M amino acids mix (glutamate, alanine, glycine, lysine, serine), 0.1 M HEPES/MOPS pH 7.5 {Molecular Dimensions Morpheus HT-96 Condition H8}