6U4B

WbbM bifunctional glycosytransferase apo structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-19
Detector	RAYONIX MX300HE
Wavelength(s)	0.97949
Spacegroup name	P 63
Unit cell lengths	124.030, 124.030, 73.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.280 - 2.100
R-factor	0.1902
Rwork	0.188
R-free	0.22690
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.523
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.288	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	37124	2755
<I/σ(I)>	14.7	2.05
Completeness [%]	99.0
Redundancy	5.7
CC(1/2)		0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	50 mM MgCl, 0.1 M HEPES and 30% v/v PEG MME 550