6U47
Coiled-coil Trimer with Glu:Ala:Lys Triad
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-07 |
Detector | APEX II CCD |
Wavelength(s) | 1.5406 |
Spacegroup name | H 3 |
Unit cell lengths | 39.015, 39.015, 98.163 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.856 - 2.301 |
R-factor | 0.19 |
Rwork | 0.183 |
R-free | 0.24410 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2906) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.856 | 2.383 |
High resolution limit [Å] | 2.301 | 2.301 |
Rmerge | 0.199 | 0.364 |
Rmeas | 0.215 | 0.416 |
Rpim | 0.080 | 0.200 |
Number of reflections | 4941 | 240 |
<I/σ(I)> | 8.5 | 1.6 |
Completeness [%] | 99.6 | |
Redundancy | 5.8 | |
CC(1/2) | 0.892 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | 50% PEG200, 100 mM sodium phosphate dibasic/potassium phosphate monobasic, pH 6.2 |