6U3V

Crystal structure of human alpha/epsilon-COP of the COPI vesicular coat bound to alpha-COP STM1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97933
Spacegroup name	P 31 2 1
Unit cell lengths	138.103, 138.103, 192.940
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.547 - 2.960
R-factor	0.1748
Rwork	0.172
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tzt
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.13_2998: ???)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.550	3.030
High resolution limit [Å]	2.956	2.960
Rmerge	0.114	0.954
Rmeas	0.120	1.005
Rpim	0.038	0.313
Total number of observations		31338
Number of reflections	45037	3157
<I/σ(I)>	16.6	2.3
Completeness [%]	99.6	96.3
Redundancy	10.1	9.9
CC(1/2)	0.999	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	300	0.1 M sodium citrate, pH 6.0, 0.05% (v/v) Tacsimate, pH 5.0, 11% (w/v) PEG 3350, 5 mM dithiothreitol Cryoprotected with 30% (v/v) ethylene glycol