6U3D
1.75 Angstrom crystal structure of the N53I Ca-CaM:CaV1.2 IQ domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.033200 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.400, 43.369, 66.962 |
Unit cell angles | 90.00, 111.93, 90.00 |
Refinement procedure
Resolution | 35.560 - 1.750 |
R-factor | 0.1654 |
Rwork | 0.164 |
R-free | 0.20090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6daf |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 35.560 | 1.860 |
High resolution limit [Å] | 1.750 | 5.200 | 1.750 |
Number of reflections | 56097 | 2179 | 7029 |
<I/σ(I)> | 11.55 | ||
Completeness [%] | 89.6 | ||
Redundancy | 1.72 | ||
CC(1/2) | 0.997 | 0.997 | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350, citric acid |