6U3B

1.7 Angstrom crystal structure of the Q135P Ca-CaM:CaV1.2 IQ domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 2 21
Unit cell lengths	41.611, 56.594, 68.586
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.576 - 1.700
R-factor	0.1726
Rwork	0.171
R-free	0.20040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6daf
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	35.576	1.800
High resolution limit [Å]	1.700	5.050	1.700
Number of reflections	18481	798	2927
<I/σ(I)>	16.32
Completeness [%]	99.9
Redundancy	10.61
CC(1/2)	0.999	1.000	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 550 MME, sodium acetate