6U1Q

Crystal Structure of VpsO (VC0937) Kinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 64
Unit cell lengths	171.430, 171.430, 44.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.858 - 2.870
R-factor	0.1775
Rwork	0.175
R-free	0.22830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jlv
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.390	3.030
High resolution limit [Å]	2.870	2.870
Rmerge	0.096	0.660
Number of reflections	17506	2518
<I/σ(I)>	9.2	2.5
Completeness [%]	100.0
Redundancy	4.6
CC(1/2)	0.990	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M sodium thiocyanate, pH 6.9, 20% PEG 3350