6U0Y
Crystal Structure of the metallo-beta-lactamase L1 from Stenotrophomonas maltophilia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-28 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.033, 97.229, 91.725 |
| Unit cell angles | 90.00, 105.55, 90.00 |
Refinement procedure
| Resolution | 48.630 - 1.700 |
| R-factor | 0.1693 |
| Rwork | 0.168 |
| R-free | 0.19780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 5DPX |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.851 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.122 | 0.843 |
| Number of reflections | 122234 | 6033 |
| <I/σ(I)> | 23.1 | 2.38 |
| Completeness [%] | 96.6 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.990 | 0.735 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M HEPES pH 7.5, 10 %(w/v) PEG4000, 5 %(v/v) isopropanol |
