6U05

Crystal Structure of Fungal RNA Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	77.747, 44.690, 79.500
Unit cell angles	90.00, 93.53, 90.00

Refinement procedure

Resolution	39.675 - 1.948
R-factor	0.187
Rwork	0.185
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u32
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.948	5.290	1.950
Rmerge	0.068	0.032	0.556
Rmeas	0.078	0.037	0.663
Rpim	0.037	0.017	0.356
Number of reflections	38436	2052	1652
<I/σ(I)>	7.6
Completeness [%]	96.3	97.3	83.1
Redundancy	3.9	4.1	2.7
CC(1/2)		0.999	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M KH2PO4, 20% PEG3350