6U04

Crystal structure of human BRPF1 PZP bound to histone H3 tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-30
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 61 2 2
Unit cell lengths	72.461, 72.461, 155.297
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.684 - 2.200
R-factor	0.1756
Rwork	0.172
R-free	0.20850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5erc
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.196	4.740	2.200
Rmerge	0.144	0.066	0.794
Rmeas	0.148	0.067	0.814
Rpim	0.032	0.014	0.177
Number of reflections	12975	1446	1249
<I/σ(I)>	6
Completeness [%]	99.5	98.1	100
Redundancy	20.8	21.4	20.7
CC(1/2)		0.999	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	0.2 M Lithium sulfate, 0.1 M Tris-HCl pH 8.5, 40% (v/v) PEG400, and 0.01M Praseodymium(III) acetate hydrate